Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review
نویسندگان
چکیده
منابع مشابه
Genetic Variability of Calpastatin and Calpain Genes in Iranian Zel Sheep Using PCR-RFLP and PCR-SSCP Methods
The genotypes for calpastatin (CAST) and calpain (CAPN) loci were determined by PCR-RFLP and PCRSSCPmethods. Blood samples were collected from 200 pure-bred Zel sheep from Shirang’s Zel sheep Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic an...
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Background and Purpose: The epidemiological alteration in the distribution of Candida species, as well as the significantly increasing trend of either intrinsic or acquired resistance of some of these fungi highlights the need for a reliable method for the identification of the species. Polymerase chain reaction (PCR) is one of the methods facilitating the quick and precise identification of Ca...
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15 صفحه اولRapid identification of extensively and extremely drug resistant tuberculosis from multidrug resistant strains; using PCR-RFLP and PCR-SSCP
BACKGROUND AND OBJECTIVES Resistance in Mycobacterium tuberculosis is caused by mutations in genes encoding drug targets. Investigators have already demonstrated the existence of mutations in codons 88 to 94 in the gyrA gene and also in codons 1400, 1401, and 1483 of rrs gene among extensively and extremely drug resistant tuberculosis (XDR & XXDR-TB) strains. The aim of this study was to identi...
متن کاملDetection and isolation of nuclear haplotypes by PCR-SSCP.
SSCP (single-strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated with more conventional cloning procedures....
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ژورنال
عنوان ژورنال: Journal of Applied Biotechnology Reports
سال: 2019
ISSN: 2423-5784
DOI: 10.29252/jabr.06.04.02